Kobe University Repository : Kernel Tit le Ca 2 + - and glycoconjugates - dependent prey capture inthe
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چکیده
Exocytosis of extrusomes, secretory granules found in protozoa, is involved in prey capture by the heliozoon Actinophrys sol. Here we show that extracellular Ca 2+ is necessary for exocytosis and prey capture in A. sol. We found that A. sol could not capture prey cells in a Ca 2+-free solution. L-type Ca 2+ channel blockers and a calmodulin antagonist also inhibited the capture of prey. These results suggest that Ca 2+ influx via L-type Ca 2+ channels plays a crucial role in exocytosis in A. sol. Concanavalin A (Con A) also inhibited prey capture, and the inhibition was relieved by the addition of its hapten sugar, α-mannoside, suggesting that Con A-binding glycoconjugates are implicated in exocytosis of extrusomes and the adhesion of prey cells.
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Kobe University Repository : Kernel
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